ABSTRACT
Objective:To investigate the expression of programmed cell death protein 1 (PD-1), programmed cell death ligand 1(PD-L1), and lymphocyte-activation gene 3(LAG-3) in different subsets of lymphocytes and their relationship with the immunologic imbalance in preeclampsia.Methods:We enrolled 25 cases of singleton pregnant women with preeclampsia who were delivered by cesarean section in the Shandong Provincial Hospital Affiliated to Shandong First Medical University from May 2019 to January 2020 as the preeclampsia group. According to the allocation ratio of 1∶1 matched for the date of cesarean section and pregnancy week at delivery, another 25 healthy singleton pregnant women underwent elective cesarean section were selected as the normal group. The decidua tissue was obtained during cesarean section. The expression levels of PD-1, PD-L1, and LAG-3 on decidual T cells, natural killer (NK), and natural killer T (NKT) cells were measured by flow cytometry and compared between the two groups using two independent samples- t test. Results:(1) The expression of PD-1 on decidual T cells and NK cells of the preeclampsia group were lower than those of the normal group (37.84±3.82 vs 57.02±3.89, t=3.529, P<0.001; 3.28±0.48 vs 5.69±0.99, t=2.184, P=0.034), but did not differ significantly in the expression on decidual NKT cells ( P=0.461). PD-L1 expression on decidual NK cells of preeclampsia group was lower than that of the normal group (0.60±0.11 vs 1.32±0.19, t=3.319, P=0.002), but showed no significant difference in the expression level on T cells and NKT cells (both P>0.05). The preeclampsia group was noted for a lower expression of LAG-3 on decidual T cells and NKT cells compared with the normal group (2.32±0.36 vs 4.09±0.67, t=2.335, P=0.024; 35.40±4.97 vs 56.27±4.49, t=3.282, P=0.002), while showed no significant difference in the expression level of NK cells ( P=0.112). Conclusions:The decreased expression of PD-1, PD-L1, and LAG-3 in the decidual lymphocyte subsets may be involved in the immunologic imbalance of preeclampsia through the over-activation of immunocytes at the maternal-fetal interface.
ABSTRACT
This study aimed to evaluate the expressions and clinical significance of bone Gla-protein [BGP], bone alkaline phosphatase [B-ALP] and C-terminal telopeptide of type I collagen [CTX] in patients with osteoporosis [OP], and to provide evidence for developing individualized treatment plans. Seventy-two OP patients in our hospital were selected as an OP group, and another 72 healthy subjects were used as a control group. Their BGP, B-ALP and CTX levels as well as bone mineral density [BMD] values were measured. The correlations between BGP, B-ALP and CTX levels and BMD values were determined. The BGP level of the OP group [[5.61 +/- 5.52] ng/ml] was significantly higher than that of the control group [P<0.05], but the levels of B-ALP and CTX did not differ significantly [P>0.05]. The BMD values of femoral neck and Ward's triangle in the OP group were negatively correlated with the B-ALP levels [P<0.05]. For women OP patients, the BMD values of femoral neck and Ward's triangle were also negatively correlated with the B-ALP levels [P<0.05]. The BMD of femoral neck in the control group was negatively correlated with the CTX level [P<0.05]. Determining BGP, B-ALP and CTX levels can evaluate the bone metabolism degree, which provides evidence for clinical typing of OP and developing treatment strategies